Functional studies of plasma membrane aquaporins from Zea mays
(2002)
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- Authors
- Supervisors
- Chaumont, F.
- Abstract
- (en) Transmembrane water movement can occur by simple diffusion but it is also facilitated by water channels called aquaporins. These proteins belong to the Major Intrinsic Protein (MIP) family of intégral membrane proteins identified in vertebrates, insects, higher plants, yeast and bacteria. Analysis of 2D crystal structure shows that MIPs hâve 6 transmembrane spans connected by 5 loops (A-E). The loops B and E containing the highly conserved NPA motifs fold back into the membrane and are involved in the formation o f the pore structure and specificity. Our laboratory characterizes the function and régulation o f maize MIP proteins présent in the plasma membrane. As observed in other plant species the Plasma membrane Intrinsic Protein (PIP) family can be divided into two subgroups, PIPI and PIP2. Contrary to ZmPIPl;l and ZmPIPl;2 the expression o f ZmPIP2;5 increased significantly the osmotic water permeability (Pf) of Xenopus oocyte membrane. The dysfunction of ZmPIPls was not due to their mistargeting to the oocyte plasma membrane.
- Affiliations
Citations
Fetter, K. (2002). Functional studies of plasma membrane aquaporins from Zea mays. https://hdl.handle.net/2078.5/98380