Contribution of plasma membrane lipid domains to red blood cell (re)shaping.
(2017) Scientific Reports — Vol. 7, n° 1, p. 4264 [1-17] (2017)
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- Although lipid domains have been evidenced in several living cell plasma membranes, their roles remain largely unclear. We here investigated whether they could contribute to function-associated cell (re)shaping. To address this question, we used erythrocytes as cellular model since they (i) exhibit a specific biconcave shape, allowing for reversible deformation in blood circulation, which is lost by membrane vesiculation upon aging; and (ii) display at their outer plasma membrane leaflet two types of submicrometric domains differently enriched in cholesterol and sphingomyelin. We here reveal the specific association of cholesterol- and sphingomyelin-enriched domains with distinct curvature areas of the erythrocyte biconcave membrane. Upon erythrocyte deformation, cholesterol-enriched domains gathered in high curvature areas. In contrast, sphingomyelin-enriched domains increased in abundance upon calcium efflux during shape restoration. Upon erythrocyte storage at 4 °C (to mimick aging), lipid domains appeared as specific vesiculation sites. Altogether, our data indicate that lipid domains could contribute to erythrocyte function-associated (re)shaping.
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Léonard, C., Conrard, L., Guthmann, M., Pollet, H., Carquin, M., Vermylen, C., Gailly, P., Van Der Smissen, P., Mingeot-Leclercq, M.-P., & Tyteca, D. (2017). Contribution of plasma membrane lipid domains to red blood cell (re)shaping. Scientific Reports, 7(1), 4264 [1-17]. https://doi.org/10.1038/s41598-017-04388-z (Original work published 2017)