Evidence for glucose-6-phosphate transport in rat liver microsomes.

Gerin, Isabelle;Van Schaftingen, Emile
(2002) FEBS Letters — Vol. 517, n° 1-3, p. 257-260 (2002)

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The existence of glucose-6-phosphate transport across the liver microsomal membrane is still controversial. In this paper, we show that S3483, a chlorogenic acid derivative known to inhibit glucose-6-phosphatase in intact microsomes, caused the intravesicular accumulation of glucose-6-phosphate when the latter was produced by glucose-6-phosphatase from glucose and carbamoyl-phosphate. S3483 also inhibited the conversion of glucose-6-phosphate to 6-phosphogluconate occurring inside microsomes in the presence of electron acceptors (NADP or metyrapone). These data indicate that liver microsomal membranes contain a reversible glucose-6-phosphate transporter, which furnishes substrate not only to glucose-6-phosphatase, but also to hexose-6-phosphate dehydrogenase.
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Gerin, I., & Van Schaftingen, E. (2002). Evidence for glucose-6-phosphate transport in rat liver microsomes. FEBS Letters, 517(1-3), 257-260. https://doi.org/10.1016/S0014-5793(02)02640-6 (Original work published 2002)