High-throughput screeling of Rhizobacteria with antagonistic and elicitation activities for potato protection

Desoignies, Nicolas;Modrie, Pauline;Caulier, Simon;Liépin, Maxime;Bragard, Claude;et.al.
(2016) 19th Triennial Conference of the European Association for Potato Research 6 to 11 July 2014 — Location: Brussels, Belgium (6.July.2014)

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Authors
  • Desoignies, NicolasUCLouvain
    Author
  • Modrie, PaulineUCLouvain
    Author
  • Caulier, SimonUCLouvain
    Author
  • Liépin, MaximeUCLouvain
    Author
  • Mahillon, JacquesUCLouvain
    Author
  • Legrève, Anneorcid-logoUCLouvain
    Author
  • Bragard, Claudeorcid-logoUCLouvain
    Author
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Abstract
The increasing acreage of organic crops altogether with policies more restrictive regarding the number and quantities of pesticides available for agriculture stimulate research of new strategies for crop protection in both organic as well as integrated pest management applied to potato crops. A high throughput screening method was used to select high potential bacterial strain to be used as growth promoting rhizobacteria for putative application as biopesticide. More than 2600 bacterial strains of Pseudomonas spp. and Bacillus spp. have been collected in fifteen soils, composts and potato plants in the Waloon region. Their antagonist activity was assessed in vitro against three pathogens of potato : Phytophthora infestans, Fusarium solani and Pectobacterium carotovorum. On the 2600 selected strains, 45 Bacillus spp. and 15 Pseudomonas spp. showed direct activity against one or some of the pathogens. Such strains were further tested for their eliciting abilities. Mutant Arabidopsis were used to make a first selection between the bacterial strains : the reporter GUS fused with an Arabidopsis plant defensin gene was used to indicate the elicitation of induced systemic resistance by the tested bacteria. The Arabidopsis with the higher GUS-expression were selected and expression of selected Arabidopsis genes involved in ISR was followed by RT-qPCR. Finally, the nine best ISR-inducing bacteria were selected and further evaluated under greenhouse condition. Possibilities for large scale applications as well as putative side effects will be discussed.
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Desoignies, N., Modrie, P., Caulier, S., Liépin, M., Mahillon, J., Legrève, A., & Bragard, C. (2016). High-throughput screeling of Rhizobacteria with antagonistic and elicitation activities for potato protection. 19th Triennial Conference of the European Association for Potato Research 6 to 11 July 2014, Brussels, Belgium. https://hdl.handle.net/2078.5/255770