Mechanically micropatterned polyelectrolyte multilayers to control cell behavior
(2013)
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- Abstract
- Polyelectrolyte multilayers have emerged as a versatile tool in the field of biomaterials and tissue engineering. In this study, photocrosslinkable polyelectrolyte films based on biopolymers whose stiffness can be easily tuned by UV irradiation were prepared. Then, they were tested against bacteria and mammalian cells to address the influence of the film stiffness on cell behavior. Such superficial stiffness manipulation resulted in differential response of bacteria and mammalian cells. Gram negative bacteria evidenced better growth on softer films while various mammalian cells preferred stiffer films. Stiffness patterns of various geometries and sizes were generated by exposing the films to the UV light through a photomask incorporated in transparent substrates. The patterned films composed of stiff motifs distributed in a soft background induced a preferential spatial organization, which depended on pattern shape and size. A comparative study with commercial biochemical patterns revealed similar pattern fidelity for three different mammalian cell types. Such mechanical patterns on a 2D film appear promising for future applications in tissue engineering or for drug screening.
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Citations
Saha, N. (2013). Mechanically micropatterned polyelectrolyte multilayers to control cell behavior. https://hdl.handle.net/2078.5/25570