Particle counting immunoassay for urinary cotinine: Comparison with chromatography, enzyme-linked immunoassay and fluorescence polarization immunoassay

Galanti, Laurence;Dell'Omo, Juliette;Vanbeckbergen, Danielle;Dubois, Pierre;Cambiaso, Cesar;et.al.
(1999) Clinical Chemistry and Laboratory Medicine : Associated with FESCC and IFCC — Vol. 37, n° 7, p. 729-734 (1999)

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Authors
  • Galanti, LaurenceUCLouvain
    Author
  • Dell'Omo, Juliette
    Author
  • Vanbeckbergen, DanielleUCLouvain
    Author
  • Dubois, Pierre
    Author
  • Masson, PierreUCLouvain
    Author
  • Cambiaso, CesarUCLouvain
    Author
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Abstract
Urinary cotinine was measured according to its inhibitory activity on the agglutination of cotinine-coated latex particles by anti-cotinine antibodies, the agglutination being measured by optical counting of the remaining non-agglutinated particles (particle counting, PaC). The detection limit was 0.03 microgram/ml and the practical range extended from 0.03 to 3.9 micrograms/ml. The correlation results of 320 urine samples with those of high pressure liquid chromatography, enzyme-linked (Coti-Tracq EIA, Serex Inc., Maywood, NJ, USA), and fluorescence polarization immunoassay (TDX instrument, Abbott, Abbott Park, IL, USA) were r = 0.90, r = 0.69, r = 0.87, respectively, whereas the correlation coefficients between the assays other than particle counting ranged from 0.62 to 0.88. PaC does not require any separation step and can thus be easily automated.
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Galanti, L., Dell’Omo, J., Vanbeckbergen, D., Dubois, P., Masson, P., & Cambiaso, C. (1999). Particle counting immunoassay for urinary cotinine: Comparison with chromatography, enzyme-linked immunoassay and fluorescence polarization immunoassay. Clinical Chemistry and Laboratory Medicine : Associated with FESCC and IFCC, 37(7), 729-734. https://doi.org/10.1515/CCLM.1999.112 (Original work published 1999)