Protocol to assess the suppression of T-cell proliferation by human MDSC.
(2020) Methods in Enzymology — Vol. 632, p. 155-192 (2020)
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- Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppressor cell (MDSC) functions. However, diverse methodologies hinder the comparison of results obtained in different laboratories. In this chapter, we present a T-cell proliferation assay procedure based on allogeneic MDSC and T-cells that is potentially suitable to multi-center studies. The T-cells are isolated from non-cancerous donors and frozen for later use in different research groups. We observed that pure thawed T-cells showed poor proliferative capacities. To retain proliferation, T-cell-autologous mature dendritic cells are supplemented after thawing. MDSC are isolated from clinical samples and represent the sole variant between assays. Flow cytometry is used to assess T-cell proliferation by the dilution of a tracking dye.
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Bruger, A., Vanhaver, C., Bruderek, K., Amodio, G., Tavukçuoğlu, E., Esendagli, G., Gregori, S., Brandau, S., & van der Bruggen, P. (2020). Protocol to assess the suppression of T-cell proliferation by human MDSC. Methods in Enzymology, 632, 155-192. https://doi.org/10.1016/bs.mie.2019.05.046 (Original work published 2020)