Endoplasmic reticulum resident, immunoglobulin joining chain, can be secreted by perturbation of the calcium concentration in the endoplasmic reticulum

Asano, Masatake;Ogura, Yoshitaka;Takenouchi-Ohkubo, Nobuko;Chihaya, Hirotsugu;Moro, Itaru;et.al.
(2004) DNA and Cell Biology — Vol. 23, n° 7, p. 403-411 (2004)

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Authors
  • Asano, Masatake
    Author
  • Ogura, Yoshitaka
    Author
  • Takenouchi-Ohkubo, Nobuko
    Author
  • Chihaya, Hirotsugu
    Author
  • Vaerman, Jean-PierreUCLouvain
    Author
  • Moro, Itaru
    Author
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Abstract
We established a transient human joining (J)-chain gene expression system in the baby hamster kidney (BHK) cell. The J-chain was detected as a 29-kDa single band on Western blotting. Immunofluorescent staining of the transfectant revealed an exclusive localization of the J-chain in the endoplasmic reticulum (ER). Intracellular transport experiment revealed that incubating conditions favorable for vesicular stomatitis virus glycoprotein (VSV-G) transport did not allow the J-chain to exit from the ER. Analysis of glycosylation status of the J-chain in the transfectant was examined by tunicamycin treatment, endoglycosidase H digestion, and also by treatment with brefeldin A. It was found that an N-glycosylation consensus site of the J-chain was functional, and intracellular J-chain was endoglycosidase H sensitive. These results indicate that, in the absence of any immunoglobulin molecules, J-chain localizes exclusively in the ER. We also tested whether the J-chain could be exported from the ER by perturbing the Call concentration in the ER. Cultivation of the J-chain transfectant in the presence of ionomycin resulted in the time-dependent secretion of the J-chain. The secreted J-chain was modified by the Golgi resident glycosylation enzymes, indicating that the secreted J-chain passed through the normal exocytic pathway.
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Asano, M., Ogura, Y., Takenouchi-Ohkubo, N., Chihaya, H., Chung-Hsing, W., Ishikawa, K., Kobayashi, K., Vaerman, J.-P., & Moro, I. (2004). Endoplasmic reticulum resident, immunoglobulin joining chain, can be secreted by perturbation of the calcium concentration in the endoplasmic reticulum. DNA and Cell Biology, 23(7), 403-411. https://doi.org/10.1089/1044549041474779 (Original work published 2004)