Contribution to the rapid identification of some anaerobic bacteria
(1986)
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- Authors
- Supervisors
- Wauters, Georges
- Abstract
- Anaerobic bacteria clearly play a major role in many life threating infectious diseases, although their significance has yet to be determined in some clinical settings. Anaerobic infections can involve any anatomic region of the body, provided that conditions in the tissues are suitable (Dowell, 1975; Finegold, 1977). <BR> Anarobes are commonly involved in abscess of any organ, e.g. brain abscess, lung abscess, liver abscess, tuboovarian abscess, antibiotic-associated colitis, appendicitis, cholecystitis, chronic otitis media, crepitant and non crepitant cellutitis, dental and oral infections, endocarditis, septic arthritis, sinusitis, subdural empyema, bacteremia and other infections. <BR> The majority of anaerobic infections are caused by bacteria from endogenous source (i.e. the flora of oropharynx, nasopharyns, gastrointestinal tract, genitourinary tract, and skin). Exogenous disease caused by anaerobes include food-borne and wound botulism, gastroenteritis due to clostridium perfringens, tetanus, clostridial myonecrosis (gas gangrene), crepitant cellulitis. Maybe also some nosocomial C. difficile infections. <BR> The recently recognized syndrome of infant botulism can now be added to the exogenous infections. Factors in the pathogenesis of anaerobic infections include surgical or other trauma, poor blood supply, tissue necrosis and growth of aerobes or facultative anaerobes. All of these tend to lower the oxidation reduction potential and/or oxygen tension and held provide a favourable environment for anaerobic growth. Mixed anaerobic infections are often severe, difficult to treat, and associated with significant morbidity and mortality (Dowell, 1975). <BR> The anaerobes produce a variety of virulence factors, as reviewed by others (Finegold, 1977; Gorbach, 1979). Cancer may result form the action or carcinogens produced from steroid in the intestine by gut bacteria (Aries et al., 1969; Hill et al., 971; Borriello et al., 1983). Evaldson at al. (1982) mentioned that the role of the normal anaerobic human flora in host defence, dietary factors and pathogenesis is not well understood. <BR> Recent studies of the flora of various anatomical sites together with improving anaerobic techniques have given knowledge about the flora and to quantify it and to study its function. <BR> The major sites with a complex anaerobic flora are the mouth, the upper respiratory tract, digestive tract, and female genital tract. Thus, in recent years, anaerobic bacteria have become recognized as a major component of female genital flora, many of these anaerobes being considered potentially pathogenic and often involved in gynecology. <BR> Hence, it is important that these anaerobic bacteria must be identified accurately and rapidly. The conventional methods are time consuming to perform (24 to 48h anaerobic incubation), may required specialized equipement, and are expensive. <BR> So much work is being done on the anaerobic bacteria to consider practical aspects of classification and characterization. <BR> In this respect, rapid enzymatic reactions are promising. Large inoculum-to-subtrate ratio is used to promote the enzymatic reactiosn and enable the result to be read within a few hours, allowing a one step test for identification of certain species of anaerobic bacteria. <BR> the aim of our study is to investigate rapid methods such as some decarboxylase reactions, peptidase activity and simplified gas chromatographic procedures. All these rapid tests, in conjunction with other more conventional methods (such as indole production or others) will enable laboratory workers to report some of these pathogens on the same day on which an inoculum of the pure culture is available
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Citations
Abbasily, A. K. (1986). Contribution to the rapid identification of some anaerobic bacteria. https://hdl.handle.net/2078.5/112008