IL-4-independent regulation of in vivo IL-9 expression
(1997) Journal of Immunology — Vol. 159, n° 6, p. 2616-2623 (1997)
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- Authors
Coutelier, Jean-Paul 
- Abstract
- We focused on the role of IL-4 in the regulation of the Th2 cytokine IL-9. In vivo, IL-9 mRNA was detected in lymph nodes after immunization with soluble Ags. IL-9 expression preceded that of IL-4, and was not affected in IL-4 knockout mice. In contrast, a significant decrease of IL-9 message was observed in IL-10-deficient mice, indicating a role for this cytokine in the induction of IL-9 production. Treatment with anti-CD4 Ab and analysis of purified CD4 cells confirmed that IL-9 was produced by CD4+ cells. Moreover, similarly to what has been reported for IL-4, IL-9 message induction was strongly decreased by infection with lactate dehydrogenase-elevating virus. IL-9 mRNA was also detected after in vivo stimulation with anti-CD3 Ab. In this model, IL-9 expression followed that of IL-4, but was not reduced in IL-4-deficient mice. This contrasts with in vitro stimulation in which, as reported in humans, IL-9 expression in lymphocytes incubated with anti-CD3 Ab and costimulatory molecules appeared as a late event, and was partly dependent on IL-4. In vitro IL-9 secretion was reduced significantly by addition of anti-IL-4 Ab, as well as in lymphocytes from IL-4 gene-deficient mice. Taken together, our results indicate that the Th2 cytokine IL-9 can be expressed by both IL-4-dependent and -independent pathways.
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Citations
Monteyne, P., Renauld, J.-C., Van Broeck, J., Dunne, D. W., Brombacher, F., & Coutelier, J.-P. (1997). IL-4-independent regulation of in vivo IL-9 expression. Journal of Immunology, 159(6), 2616-2623. https://hdl.handle.net/2078.5/135791 (Original work published 1997)