Survival and viability of fresh and frozen-thawed in vitro bovine blastocysts.
(1995) Reproduction, Nutrition, Development — Vol. 35, n° 1, p. 3-10 (1995)
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- Abstract
- Three experiments were undertaken to investigate the effect of some factors on survival and viability of bovine blastocysts produced in vitro in serum-free oviduct-conditioned media. In vitro survival to freezing was higher for blastocysts appearing on days 6 and 7 than for day 8 blastocysts (67 and 50% hatching rate vs 12%). Transfer success of day 8 blastocysts to recipient heifers was 35% (9/26) calves born vs 19% (4/21) for cows. Embryo mortality occurred mainly during the peri-implantation period (between days 21 and 45) for cows compared with heifers. Calving rate after direct transfer of frozen blastocysts without dilution was 24% (4/17) compared with 31% (5/16) for control unfrozen blastocysts.
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Massip, A., Mermillod, P., Van Langendonckt, A., Touze, J. L., & Dessy, F. (1995). Survival and viability of fresh and frozen-thawed in vitro bovine blastocysts. Reproduction, Nutrition, Development, 35(1), 3-10. https://doi.org/10.1051/rnd:19950101 (Original work published 1995)