Topical gene transfer into rat skin using electroporation.
(2001) Pharmaceutical Research — Vol. 18, n° 1, p. 61-66 (2001)
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- Abstract
- PURPOSE: To investigate whether electroporation can be used for topical gene delivery and for DNA expression in rat keratinocytes. METHODS: The localization of a fluorescent-labelled plasmid and the expression of a reporter gene (pEGFP-N1) coding for Green Fluorescent Protein (GFP) in stripped skin were assessed by Confocal Laser Scanning Microscopy (CLSM). RESULTS: The plasmid penetrated into the epidermis within minutes after electroporation and entered the keratinocyte cytoplasm within hours. A localized expression of GFP was observed for at least 7 days in the epidermis. Skin viability was not compromised by electroporation. CONCLUSIONS: Electroporation enhances the delivery, and hence the expression, of topically applied plasmid DNA on the skin. It could be a promising alternative method to administer DNA, particularly for DNA vaccines, in the skin in vivo.
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Citations
Dujardin, N., Van Deŕ Smissen, P., & Préat, V. (2001). Topical gene transfer into rat skin using electroporation. Pharmaceutical Research, 18(1), 61-66. https://doi.org/10.1023/A:1011026726938 (Original work published 2001)