Transfer of the genes for the StyLTI restriction-modification system of Salmonella typhimurium to strains lacking modification ability results in death of the recipient cells and degradation of their DNA.

De Backer, O;Colson, Charles
(1991) Journal of bacteriology — Vol. 173, n° 3, p. 1328-1330 (1991)

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Authors
  • De Backer, OUCLouvain
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  • Colson, CharlesUCLouvain
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Abstract
The genes encoding the restriction-modification system StyLTI of Salmonella typhimurium were inserted in vivo into the conjugative plasmid pULB21. This allowed us to transfer the StyLTI genes at a very high frequency and to monitor the fate of recipient cells after mating. Transfer of the StyLTI restriction and modification genes into a modificationless recipient was lethal and resulted in degradation of the cell's DNA. This indicates that, in contrast to any other known restriction-modification systems, StyLTI cannot be established after horizontal transfer into a naive host.
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De Backer, O., & Colson, C. (1991). Transfer of the genes for the StyLTI restriction-modification system of Salmonella typhimurium to strains lacking modification ability results in death of the recipient cells and degradation of their DNA. Journal of bacteriology, 173(3), 1328-1330. https://hdl.handle.net/2078.5/134134 (Original work published 1991)