Process for the production of alanine by recombinant microorganisms
(2003)
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- Authors
- Abstract
- The present invention relates to a process for preparing alanine, especially L-alanine, by culturing a genetically modified microorganism that is able to produce alanine from glucose, characterized in that the microorganism used is one whose native sugar metabolism has been diverted to alanine formation, especially L-alanine formation, in such a manner that essentially more than 40 %, preferably more than 60 % and more preferably more than 75 % of the carbon consumed from the medium is converted into alanine, especially via pyruvate. The microorganism is preferably a homofermentative microorganism, especially a lactic acid bacterium, more particularly (a strain of) L. lactis, and is preferably a food-grade microorganism. The invention can be used for both the in situ and the stereospecific preparation of alanine. The invention also relates to the microorganisms and recombinant DNA molecules that can be used in the invention and to the alanine and alanine-containing (food) products obtained according to the invention.
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Citations
Hols, P., Kleerebezem, M., Hugenholtz, J., Ferain, T., Kuipers, O., Delcour, J., & De Vos, W. M. (2003). Process for the production of alanine by recombinant microorganisms. https://hdl.handle.net/2078.5/255397