Isolation and characterization of 29 and 19 microsatellite loci from two deep-sea luminous lanternsharks, Etmopterus spinax and Etmopterus molleri (Squaliformes, Etmopteridae)

Oury, Nicolas;Duchatelet, Laurent;Mallefet, Jérôme;Magalon, Hélène
(2019) Molecular Biology Reports : an international journal on molecular and cellular biology — Vol. 46, p. 1357-1362 (2019)

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  • Oury, Nicolasorcid-logoUMR ENTROPIE (Université de La Réunion, IRD, CNRS), Faculté des Sciences et Technologies Université de La Réunion St Denis France
    Author
  • Duchatelet, LaurentUCLouvain
    Author
  • Mallefet, JérômeUCLouvain
    Author
  • Magalon, HélèneUMR ENTROPIE (Université de La Réunion, IRD, CNRS), Faculté des Sciences et Technologies Université de La Réunion St Denis France
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Abstract
Etmopterus spinax (Linnaeus, 1758) and Etmopterus molleri (Whitley, 1939) are two bioluminescent deep-sea sharks, usually caught in large numbers as bycatch by deep-water fisheries. Yet, no study has ever involved population status of these two species using genetic tools. In order to investigate population genetic structure, diversity and connectivity of these two lanternsharks, 29 and 19 microsatellite loci were isolated from E. spinax DNA library for E. spinax and E. molleri, respectively. These loci were tested on 32 E. spinax individuals from the North Sea and seven E. molleri from the East China Sea. The number of alleles per locus ranged from 2 to 13. The observed heterozygosity ranged from 0.031 to 0.839 for E. spinax and from 0.000 to 1.000 for E. molleri, while the expected heterozygosity ranged from 0.031 to 0.903 and from 0.143 to 0.821, respectively. Almost all loci (24 and 16, respectively) were at Hardy-Weinberg equilibrium for both species and no linkage disequilibrium among loci was detected. These loci represent useful tools to better understand the population structure of these two species. Besides, they could also be suitable for other lanternsharks in general, as these latter remain largely understudied, specially in terms of understanding the basic science that will serve into their conservation.
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Oury, N., Duchatelet, L., Mallefet, J., & Magalon, H. (2019). Isolation and characterization of 29 and 19 microsatellite loci from two deep-sea luminous lanternsharks, Etmopterus spinax and Etmopterus molleri (Squaliformes, Etmopteridae). Molecular Biology Reports : an international journal on molecular and cellular biology, 46, 1357-1362. https://doi.org/10.1007/s11033-018-04578-6 (Original work published 2019)