Ru-TAP complexes and DNA: from photo-induced electron transfer to gene photo-silencing in living cells
(2013) Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences — Vol. 371, p. 20120131 (2013)
Files
No attached file found for this publication.
Details
- Authors
Marcélis, Lionel 
- Abstract
- In this review, examples of applications of the photo-induced electron transfer (PET) process between photo-oxidizing Ru–TAP (TAP = 1,4,5,8-tetraazaphenanthrene) complexes and DNA or oligodeoxynucleotides (ODNs) are discussed. Applications using a free Ru–TAP complex (not chemically anchored to an ODN) are first considered. In this case, the PET gives rise to the production of an irreversible adduct of the Ru complex on a guanine (G) base, with formation of a covalent bond. After absorption of a second photon, this adduct can generate a bi-adduct, whereby the same complex binds to a second G moiety. These bi-adduct formations are responsible for photo-cross-linking between two strands of a duplex, each containing a G base, or between two G moieties of a single strand such as a telomeric sequence, as demonstrated by polyacrylamide gel electrophoresis analyses or mass spectrometry. Scanning force microscopy also allows the detection of such photobridgings with plasmid DNA. Other applications, for example with Ru–ODN, i.e. ODN with chemically anchored Ru–TAP complexes, are also discussed. It is shown that such Ru–ODN probes containing a G base in their own sequences are capable of photo-cross-linking selectively with their targeted complementary sequences, and, in the absence of such targets, they self-photo-inhibit. Such processes are applied successfully in gene photo-silencing of human papillomavirus cancer cells.
- Affiliations
ULB
Citations
Marcélis, L., Moucheron, C., & Kirsch-De Mesmaeker, A. (2013). Ru-TAP complexes and DNA: from photo-induced electron transfer to gene photo-silencing in living cells. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, 371, 20120131. https://doi.org/10.1098/rsta.2012.0131 (Original work published 2013)