Identification de facteurs antiviraux induits par les interférons de type I et sous-exprimés dans les neurones
(2014)
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- Authors
- Supervisors
- Michiels, Thomas
- Abstract
- Type I interferons (IFN-I) are essential for organisms survival upon viral infection. During infection of the central nervous system (CNS) by neurotropic viruses, resident cells are mostly responsible for local IFN-I production. This IFN-I is crucial to restrict viral replication and spread awaiting the development of adaptive immunity that allows the clearance of the virus. Virtually all CNS cells can produce and respond to IFN-I. Firstly, we studied the specificity of neuronal response to IFN-I. In our laboratory, Sophie Paul had previously observed that mouse neurons treated with IFN-I remain susceptible to infection by two neurotropic viruses, Theiler's virus and vesicular stomatitis virus (VSV), unlike other cell types. We demonstrated that neurons readily respond to IFN-I treatment by expressing antiviral genes. However, they display a specific gene expression signature. We determined that 15 genes, induced by IN-I in fibroblasts, are weakly or not expressed in neurons in response to IFN-I. Among those genes, ApoL9b codes for an apolipoprotein L that exerts an antiviral activity against Theiler’s virus when ectopically expressed in neurons. The low expression of ApoL9 in IFN-treated neurons likely contributes to the high susceptibility of these cells to viral infection.
- Affiliations
Citations
Kreit, M. (2014). Identification de facteurs antiviraux induits par les interférons de type I et sous-exprimés dans les neurones. https://hdl.handle.net/2078.5/194273