Preliminary study of short-term preservation of ovine ovarian tissue containing preantral follicles in saline solution or TCM199
(2002) The Veterinary Record — Vol. 151, n° 1, p. 452-453 (2002)
Files
Andradeetal2002-VetRecord.pdf
Closed Access - Adobe PDF
- 899.72 KB
Details
- Authors
- Abstract
- PREANTRAL follicles represent 90 per cent of the follicular population of mammalian ovaries (Roy and Treacy 1993). However, the vast majority (99.9 per cent) become atretic during their growth and maturation in vivo (Carroll and others 1990). The maintenance of follicular quality in vitro by appropriate handling of the ovaries during their collection and transportation is important for providing healthy oocytes for cryopreservation and/or in vitro culture, as ovarian donors are frequently some distance from specialised laboratories. Several studies have successfully used saline solution to preserve bovine (Schernthaner and others 1997) and caprine (Lucci and others 1999) ovaries for a period of a few hours. Tissue culture medium 199 (TCMl99; Sigma) has also been used successfully in the transport of bovine ovaries (Figueiredo and others 1993) and bovine oocytes from antral follicles (Twagiramungu and others 1998). The aim of this study was to evaluate the effect of 0 9 per cent saline solution or TCM199 on the short-term preservation of sheep preantral follicles inside ovarian tissue, at different temperatures and over different periods of storage.
- Affiliations
Citations
Andrade, E., Andrade Amorim, C., Costa, S. H., Ferreira, M., Rodrigues, A. P., Dode, M., & Figueiredo, J. R. (2002). Preliminary study of short-term preservation of ovine ovarian tissue containing preantral follicles in saline solution or TCM199. The Veterinary Record, 151(1), 452-453. https://doi.org/10.1136/vr.151.15.452 (Original work published 2002)