The Topology of Cu-a in Relation To the Other Metal Centers in Cytochrome-c-oxidase of Saccharomyces-cerevisiae As Determined By Analysis of 2nd-site Reversions

Meunier, B.;Colson, AM.;Rich, PR.
(1995) BBA - Protein Structure and Molecular Enzymology — Vol. 1253, n° 1, p. 13-15 (1995)

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  • Meunier, B.
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  • Colson, AM.
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  • Rich, PR.
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Abstract
Second-site revertants were selected from a respiratory-deficient mutant carrying the mutation D369N located in a loop between helices IX and X close to H376 and H378, the proposed ligands of haem a(3) and haem a, respectively. A reversion was observed in subunit II, in the vicinity of the Cu-A ligands. This same reversion compensates the subunit I deficiency mutation, S140L, assumed to be near H62, the second putative histidine ligand to haem a. These data enable us to propose a three-dimensional topology in which Cu-A in subunit II is located on top of the Positive-side of subunit I and in proximity to all three of its metal centres.
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Meunier, B., Colson, AM., & Rich, PR. (1995). The Topology of Cu-a in Relation To the Other Metal Centers in Cytochrome-c-oxidase of Saccharomyces-cerevisiae As Determined By Analysis of 2nd-site Reversions. BBA - Protein Structure and Molecular Enzymology, 1253(1), 13-15. https://doi.org/10.1016/0167-4838(95)92373-4 (Original work published 1995)