Lysis of human chondrosarcoma cells by cytolytic T lymphocytes recognizing a MAGE-A3 antigen presented by HLA-A1 molecules
(2007) Journal of Orthopaedic Research : a journal for musculoskeletal investigations — Vol. 25, n° 5, p. 678-684 (2007)
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- Abstract
- Treatment of chondrosarcomas is limited to resection because these tumors are unresponsive to standard adjuvant treatments, such as chemotherapy and radiation. We have previously shown that high-grade chondrosarcomas express unspecified members of the Melanoma Antigen (MAGE) gene family. We show here that FS human chondrosarcoma (FS) cells express MAGE-A3 gene and HLA-Al molecules. In vitro assays show that a cytolytic T-lymphocyte clone (CTL) specific for a MAGE-A3 peptide presented by HLA-Al specifically lysed FS chondrosarcoma cells. Addition of antigenic peptide did not increase the susceptibility of FS cells to CTL mediated lysis, suggesting that HLA-Al expression by the chondrosarcoma cells limited their susceptibility to lysis by the anti-MAGE-A3 CTL clone. Incubation of FS cells with 50 U/mL interferon- increased surface expression of HLA class-I molecules, increased their susceptibility to lysis, and had no effect on MAGE-A3 gene expression. These results suggest that immunotherapy targeted against chondrosarcoma cells is possible. (c) 2007 Orthopaedic Research Society.
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Bluman, E. M., Coulie, P., Sun, X., Machan, J., Lin, C., Meitner, P. A., Block, J. A., & Terek, R. M. (2007). Lysis of human chondrosarcoma cells by cytolytic T lymphocytes recognizing a MAGE-A3 antigen presented by HLA-A1 molecules. Journal of Orthopaedic Research : a journal for musculoskeletal investigations, 25(5), 678-684. https://doi.org/10.1002/jor.20368 (Original work published 2007)