Bacterial communication detection in bacterial diseases of Banana plants (Musa AAB) and Bell Peppers (Capsicum annuum L)
(2008) The 12th International Symposium on Microbial Ecology - ISME12 — Location: Cairns, Australia (17.August.2008)
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- Abstract
- A large number of Gram-negative plant pathogenic bacteria have a group behavior mediated by intercellular communication which is dependent of the population density, know as quorum sensing (QS) and generally is mediated by the secretion of N-acyl-homoserine lactones (AHLs), which control a large number of bacterial functions. At the present, the plant pathogenic bacteria characterization follows routines of isolation and culture in semi-selective media for they identification. These types of routines disparage de bacterial community present in bacteriosis. This work tried to determinate if there is a bacterial community that is working as a team, where QS signals are important. Samples of banana (Musa AAb) and Bell Peppers (Capsicum annuum L.) were recollected and processed for bacteria isolation and purification. All isolated were tested for AHLs production in LB medium and in AB minimal medium using the reporter strains Chromobacterium violaceum (CV026) and Agrobacterium tumefaciens (NTL4(pZLR4)) for short and long AHLs chains, respectively. Additionally, hypersensitivity reaction (HR) test in tobacco (Nicotiana tabacum) were done. Screenings for biofilm formation in some of these bacteria were made. From banana bacteriosis were obtained 4 isolates, grouped in 2 different phenotypic categories. The bell pepper bacteriosis produced 5 different phenotypic categories of isolates; 4 of them presented QS signals for short AHLs chains, but only one showed a positive reaction for HR. The biofilm tests suggested that no-pathogenic bacteria produce biofilms although some pathogenic bacteria don’t do it. These results open an alternative to study the pathogenicity of plant bacteria with a community approach.
- Affiliations
Universidad Simón Bolívar Lab. de Biotecnología Agrícola, Dpto. de Biología Celular
Citations
Gillis, A., Santana, M. A., & Rodríguez, M. (2008). Bacterial communication detection in bacterial diseases of Banana plants (Musa AAB) and Bell Peppers (Capsicum annuum L). The 12th International Symposium on Microbial Ecology - ISME12, Cairns, Australia. https://hdl.handle.net/2078.5/195049