Cryopreservation of bovine oocytes: current status and recent developments.
Massip, Alban
(2003) Reproduction, Nutrition, Development — Vol. 43, n° 4, p. 325-330 (2003)
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Massip, AlbanUCLouvain
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Abstract
The cryopreservation of oocytes of most animal species remains a challenge due to their complex structure. Equilibrium freezing is not satisfactory because oocytes seem to be damaged by exposure for several minutes to temperatures near 0 degrees C. Therefore, cryopreservation of oocytes by vitrification--especially the use of very high cooling rates of oocytes suspended in extremely small volumes of various cryoprotective additive modifications--seems the most appropriate method.
Massip, A. (2003). Cryopreservation of bovine oocytes: current status and recent developments. Reproduction, Nutrition, Development, 43(4), 325-330. https://doi.org/10.1051/rnd:2003024 (Original work published 2003)