Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area.
(2020) Frontiers in Cellular and Infection Microbiology — Vol. 10, p. 572909 [1-7] (2020)
Files
2020_Florio_Front_Cell_Infect_Microbiol.pdf
Open Access - Adobe PDF
- 603.9 KB
Details
- Authors
- Abstract
- Several MALDI-TOF MS-based methods have been proposed for rapid detection of antimicrobial resistance. The most widely studied methods include assessment of β-lactamase activity by visualizing the hydrolysis of the β-lactam ring, detection of biomarkers responsible for or correlated with drug-resistance/non-susceptibility, and the comparison of proteomic profiles of bacteria incubated with or without antimicrobial drugs. Antimicrobial-resistance to a number of antibiotics belonging to different classes has been successfully tested by MALDI-TOF MS in a variety of clinically relevant bacterial species including members of family, non-fermenting Gram-negative bacteria, Gram-positive cocci, anaerobic bacteria and mycobacteria, opening this field to further clinically important developments. Early detection of drug-resistance by MALDI-TOF MS can be particularly helpful for clinicians to streamline the antibiotic therapy for a better outcome of patients with systemic infection, in all cases where a prompt and effective antibiotic treatment is essential to preserve organ function and/or patient survival.
- Affiliations
Citations
Florio, W., Baldeschi, L., Rizzato, C., Tavanti, A., Ghelardi, E., & Lupetti, A. (2020). Detection of Antibiotic-Resistance by MALDI-TOF Mass Spectrometry: An Expanding Area. Frontiers in Cellular and Infection Microbiology, 10, 572909 [1-7]. https://doi.org/10.3389/fcimb.2020.572909 (Original work published 2020)