Compromised humoral and delayed-type hypersensitivity responses in IL-23-deficient mice.
(2004) Journal of Immunology — Vol. 172, n° 5, p. 2827-2833 (2004)
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- Abstract
- The heterodimeric cytokine IL-23 consists of a private cytokine-like p19 subunit and a cytokine receptor-like subunit, p40, which is shared with IL-12. Previously reported IL-12p40-deficient mice have profound immune defects resulting from combined deficiency in both IL-12 and IL-23. To address the effects of specific IL-23 deficiency, we generated mice lacking p19 by gene targeting. These mice display no overt abnormalities but mount severely compromised T-dependent humoral immune responses. IL-23p19(-/-) mice produce strongly reduced levels of Ag-specific Igs of all isotypes, but mount normal T-independent B cell responses. In addition, delayed type hypersensitivity responses are strongly impaired in the absence of IL-23, indicating a defect at the level of memory T cells. T cells stimulated with IL-23-deficient APCs secrete significantly reduced amounts of the proinflammatory cytokine IL-17, and IL-23-deficient mice phenotypically resemble IL-17-deficient animals. Thus, IL-23 plays a critical role in T cell-dependent immune responses, and our data provide further support for the existence of an IL-23/IL-17 axis of communication between the adaptive and innate parts of the immune system.
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Ghilardi, N., Kljavin, N., Chen, Q., Lucas, S., Gurney, A. L., & De Sauvage, F. J. (2004). Compromised humoral and delayed-type hypersensitivity responses in IL-23-deficient mice. Journal of Immunology, 172(5), 2827-2833. https://hdl.handle.net/2078.5/233354 (Original work published 2004)