Analysis of Antigens Recognized On Human-melanoma Cells By A2-restricted Cytolytic T-lymphocytes (ctl)
(1993) International Journal of Cancer — Vol. 55, n° 2, p. 237-244 (1993)
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- We have pursued our analysis of potential tumor-rejection antigens recognized on human melanoma by autologous cytolytic T lymphocytes (CTL). We reported previously that 3 distinct antigens (A,B,C) were recognized on melanoma cell line SK29-MEL in association with HLA-A2. Selection for melanoma-cell variants resistant to anti-A CTL revealed that antigen A consists of at least 2 determinants (Aa, Ab) which can be lost separately. Genetic linkage between Aa and Ab was suggested by concomitant loss of Aa and Ab in an immunoselected tumor-cell variant. This variant was also resistant to an autologous CTL clone restricted by HLA-B45, indicating that this CTL may also recognize a determinant of antigen A. Of 11 allogeneic HLA-A2 melanoma cell lines that were tested, 5 expressed both Aa and Ab, I expressed only Aa, and 1 only Ab. None of them was lysed by anti-B or anti-C CTL clones. A CTL clone derived from another HLA-A2-melanoma patient was found to have exactly the same lytic pattern as the anti-Ab CTL of the first patient. This suggested that it may be possible to elicit an anti-Ab response in many HLA-A2 patients. We conclude that there are at least 2 distinct antigens presented in association with HLA-A2 that are common to many melanomas and therefore constitute promising targets for specific immunotherapy. (C) 1993 Wiley-Liss, Inc.
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Wolfel, T., Hauer, M., Klehmann, F., Brichard, V., Ackermann, B., Knuth, A., Boon, T., & Zumbuschenfelde, KHM. (1993). Analysis of Antigens Recognized On Human-melanoma Cells By A2-restricted Cytolytic T-lymphocytes (ctl). International Journal of Cancer, 55(2), 237-244. https://doi.org/10.1002/ijc.2910550212 (Original work published 1993)