Identification and Characterization of a Rhizobium-leguminosarum Bv Phaseoli Gene That Is Important for Nodulation Competitiveness and Shows Structural Homology To a Rhizobium-fredii Host-inducible Gene

Michiels, J.;Pelemans, H.;Vlassak, K.;Verreth, C.;Vanderleyden, Jos
(1995) Molecular Plant - Microbe Interactions — Vol. 8, n° 3, p. 468-472 (1995)

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  • Michiels, J.
    Author
  • Pelemans, H.
    Author
  • Vlassak, K.
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  • Verreth, C.
    Author
  • Vanderleyden, JosUCLouvain
    Author
Abstract
DNA sequence analysis of a 1.4-kb SalI-HindIII segment located approximately 2 kb upstream of the Rhizobium leguminosarum bv. phaseoli syrM gene revealed the presence of an open reading frame (ORF3) encoding a putative 295-amino acid polypeptide with a molecular mass of 33,401 Da. ORF3 is homologous to a R. fredii host-inducible gene. The proteins encoded by R. l. bv. phaseoli ORF3 and by the R. fredii host-inducible gene share 37% sequence identity. In contrast to the R. fredii host-inducible gene, expression of ORF3 is not induced in the presence of Phaseolus vulgaris root exudates or by specific flavonoids, able to induce nodulation genes in R. l. bv. phaseoli. A R. l. bv. phaseoli ORF3 mutant was constructed by site-directed deletion/replacement mutagenesis. This mutant strain is not affected in symbiotic nitrogen fixation but exhibits a delay in nodulation on Phaseolus vulgaris. Moreover, this mutant was shown to be defective in competition for nodulation.
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Michiels, J., Pelemans, H., Vlassak, K., Verreth, C., & Vanderleyden, J. (1995). Identification and Characterization of a Rhizobium-leguminosarum Bv Phaseoli Gene That Is Important for Nodulation Competitiveness and Shows Structural Homology To a Rhizobium-fredii Host-inducible Gene. Molecular Plant - Microbe Interactions, 8(3), 468-472. https://hdl.handle.net/2078.5/84900 (Original work published 1995)