Macroporous scaffolds for tissue engineering : 3D printing & modification of the surface mechanical properties
(2019)
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- Abstract
- Additive manufacturing techniques are promising technologies to produce patient-specific and effective scaffolds for tissue engineering. Chemical and physical surface modifications are often performed on scaffolds to control cell behavior and foster tissue regeneration. On the other hand, surface mechanical properties are known to influence stem cell proliferation and to direct their differentiation. The aim of this work was to produce degradable poly(lactic acid) (PLA) and poly(epsilon-caprolactone) scaffolds using a so-called gyroid geometry, which features an isotropic and spring-shaped design. This was achieved by adapting the parameters to get closer to the resolution limit of fused deposition modeling, an easy-to-use and low-cost 3D printing technology. A semi-interpenetrating polymer network made with poly(dimethylsiloxane) crosslinked at different densities was then designed to modify the surface mechanical properties of PLA gyroid scaffolds. Finally, the effect of the coating mechanical properties on the proliferation and osteogenic differentiation of stem cells from the apical papilla was evaluated. The production of complex scaffolds was successfully achieved using 3D printing. The coating technology that was further developed paves the way towards the modification of the surface mechanical properties of macroporous scaffolds with a complex shape, with a view to control stem cell behavior.
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Citations
Germain, L. (2019). Macroporous scaffolds for tissue engineering : 3D printing & modification of the surface mechanical properties. https://hdl.handle.net/2078.5/124650