Development of a new EPR tool to detect mitochondrial superoxide
(2019)
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- Abstract
- Mitochondrial superoxide is widely investigated as its implication was suggested in the development of neurodegenerative diseases, hypertension and cancer. So far, only a few techniques exist to detect this species sensitively and specifically. Fluorescent-based probes (e.g., mitoSOX) are the most widely used tools. However, several concerns about their specificity were highlighted further questioning the validity of the information provided. Electron Paramagnetic Resonance (EPR) is a technique of choice to detect and identify the radicals involved in a given biological process. The aim of the thesis was to develop a new EPR probe targeted to mitochondria. Different EPR techniques exist although they have never been systematically compared to each other. The first study allowed us to select CMH as the most sensitive tool to detect superoxide. Then, we targeted the latter to mitochondria by linking a triphenylphosphonium moiety to form mitoCPH. We next characterized this tool in PBS, lysates and paraquat-stimulated cells. Finally, we attempted to detect mitochondrial superoxide levels in superinvasive cancerous cell lines.
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Citations
Scheinok, S. (2019). Development of a new EPR tool to detect mitochondrial superoxide. https://hdl.handle.net/2078.5/91549