Evaluation of the activity of antibiotics and adjuvant strategies against dual species biofilms in the context of cystic fibrosis
(2024)
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- Authors
- Supervisors
- van bambeke, Françoise
- Abstract
- Cystic fibrosis (CF) is an inherited multiorgan disease characterised by thick mucus in the airways. The morbidity and mortality of this disease are mainly related to pulmonary bacterial infections. S. aureus and P. aeruginosa are the most predominant pathogens and both species are often isolated from the same patients. This work aims to develop dual-species biofilms of S. aureus and P. aeruginosa that mimic in vivo growth conditions, and examine whether selected enzymes or phages can improve antibiotic activity against these dual-species biofilms. We first showed a cross-adaptation between co-isolates from both species coming from the same patients allowing them to form a stable dual species biofilm. We demonstrated that DNase I and alginase are useful adjuvants to antibiotics to improve efficacy against dual-species biofilm of clinical isolates. Phages also demonstrated their interest as adjuvants to antibiotics but were tested so far only against biofilms from reference strains.
- Affiliations
Citations
Wang, Z. (2024). Evaluation of the activity of antibiotics and adjuvant strategies against dual species biofilms in the context of cystic fibrosis.