Colistin-resistant genes mcr-1 to -5, including a case of triple occurrence (mcr-1, -3, -5), in E. coli isolates from faeces of healthy pigs, cattle and poultry in Belgium between 2012 and 2016.

Timmermans, Michaël;Wattiau, Pierre;Denis, Olivier;Boland, Cécile
(2021) International Journal of Antimicrobial Agents — Vol. 57, n° 6, p. 106350 [1-9] (2021)

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Authors
  • Timmermans, Michaël
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  • Wattiau, Pierre
    Author
  • Denis, OlivierUCLouvain
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  • Boland, Cécile
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Abstract
Colistin is a last resort antimicrobial used to treat infections caused by multi-drug resistant Gram-negative rods (MDRGNR). The emergence of colistin resistance, particularly linked to mobile genetic elements including mcr genes is a major threat to the management of MDRGNR infections. The aim of this study was to assess the presence of mcr genes in a collection of 40 colistin-resistant commensal Escherichia coli isolated from healthy pigs, cattle and poultry in Belgium between 2012 and 2016. All isolates carried at least one mcr gene. mcr-1 to -5 were observed in this collection. Different replicons associated with mcr genes were identified: IncHI2/IncHI2A associated with mcr-1, IncX4 with mcr-1 and mcr-2 and ColE10 with mcr-4. While multiple mcr genes occurrences in a single isolate have rarely been reported elsewhere, a triple occurrence (mcr-1, -3, -5) was found in this study. All isolates were multi-drug resistant and carried between 1 and 9 different replicons. Seventeen different sequence types were observed among the 40 E. coli isolates. In conclusion, this study revealed the presence of a reservoir of mobile resistant genes to colistin (mcr-1 to mcr-5) observed during at least 5 years (2012 - 2016) in the commensal gut flora of pigs, cattle and poultry.
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Timmermans, M., Wattiau, P., Denis, O., & Boland, C. (2021). Colistin-resistant genes mcr-1 to -5, including a case of triple occurrence (mcr-1, -3, -5), in E. coli isolates from faeces of healthy pigs, cattle and poultry in Belgium between 2012 and 2016. International Journal of Antimicrobial Agents, 57(6), 106350 [1-9]. https://doi.org/10.1016/j.ijantimicag.2021.106350 (Original work published 2021)