Anti-Interleukin-9 vaccination: a new tool to dissect IL-9 activities in vivo
(2002)
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- Abstract
- (en) Interleukin 9 (IL-9) is a multifunctional cytokine produced by activated Th2 clones in vitro and during Th2- Iike T cell responses in vivo. Although IL-9 was initially described as a T cell growth factor, its role in T cell responses is still unclear. Other biological targets for IL-9 include B lymphocytes, eosinophils, mast cells, epithelial cells and hematopoietic progenitors. The purpose of this work was to develop a tool to analyze IL-9 effects in vivo. To this end, mice were immunized with mouse IL-9 coupled to a non-self protein. In all mouse strains tested, this procedure generated strong and long-lasting anti-IL-9 auto-antibody production. This immunization completely abrogated the increase in mast cell protease-1 (MMCP-I) levels as well as the eosinophilia observed in mice after implantation of an IL-9-secreting cell line. We thus took advantage of this method to assess the role of IL-9 in infections with nematode Trichuris muris, where IL-9 production correlates with a resistant phenotype. C57BI∕6 mice, which normally expel the parasite, became susceptible after anti-IL-9 immunization, demonstrating that IL-9 plays a critical role in this model. In addition, neutralization of IL-9 also inhibited parasite-induced blood eosinophilia. Taken together, this work demonstrates the potency of our novel strategy to antagonize IL-9 in vivo and shows that this cytokine plays a major role in resistance against T. muris infection.
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Richard, M. (2002). Anti-Interleukin-9 vaccination: a new tool to dissect IL-9 activities in vivo. https://hdl.handle.net/2078.5/110903