Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion.
(2008) Journal of Biotechnology — Vol. 139, n° 4, p. 291-299 (2009)
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- Authors
Agathos, Spiros N. 
- Abstract
- Bone marrow mesenchymal stem cells (BM-MSCs) have the potential to be used for tissue engineering. Nevertheless, they exhibit a low growth rate that limits their availability. In this work we use an alternative model of MSC from the outer ear (ear mesenchymal stem cells, E-MSC). These cells bear the characteristics of progenitor cells because of their ability to be differentiated into the three lineages of chondrocytes, osteocytes and adipocytes. This model cell population had a threefold higher cell growth rate compared to BM-MSC. This allowed rapid testing of the scalability in microcarrier culture using bead-to-bead transfer and also enabled their expansion in a 1-l bioreactor. The cells were able to maintain their potential for differentiation into the above three lineages. Therefore, E-MSC appear to be an attractive model for assessing a number of bioengineering parameters that may affect the behavior of adult stem cells in culture.
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Citations
Sart, S., Schneider, Y.-J., & Agathos, S. N. (2008). Ear mesenchymal stem cells: An efficient adult multipotent cell population fit for rapid and scalable expansion. Journal of Biotechnology, 139(4), 291-299. https://doi.org/10.1016/j.jbiotec.2008.12.011 (Original work published 2009)