Effect of exposure to Bisphenol A in the thickness of uterine layers in the vesper mouse
(2020) The 19th World Congress of the International Society of Gynecological Endocrinology — Location: Online (2.December.2020)
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- Abstract
- Context: It is well known that Bisphenol A (BPA) is a substance used in the production of polycarbonate and epoxy resins that acts as an endocrine disruptor. The vesper mouse (Calomys laucha) is a small wild rodent found in South America, which is used in research for its genetic variability and resistance to stressful agents. As it lives on coasts, pastures and along roadsides, it may be affected by environmental sources of BPA and therefore act as a bioindicator for the effects of endocrine disruptors in humans. Objective: The goal of this study was to verify the effects of BPA in litter parameters and uterine layer thickness of C. laucha. Methods: Sixty C. laucha females of 50-70 days old received by gavage during pregnancy the following substances: olive oil (negative control) or BPA (40, 80 or 200 μg/Kg). After birth, pups were counted and sexed. Mothers were euthanized after lactation period. Female offspring were euthanized on PND 70, after reaching full maturation of the reproductive tract. Uteri were processed for histology. Measurements for myometrium, endometrium and total uterine thickness (n=10 mothers and 10 offspring) were performed using ImageJ software. Results: Of all sixty females, 41 sired litters. One animal from the BPA40 group was born dead and without three limbs. Two animals from the BPA80 group presented male genitals and female reproductive tract. Proportion of gender per treatment was 1:1 in all groups. Number of pups per litter showed no differences (p<0.05) between the groups, with a mean of 3.8 ± 0.31 females and 2.2 ± 0.48 males. Uteri from one animal of BPA40 and four animals of BPA80 offspring groups presented edema when removed, as well as from two mothers of BPA80 group. In mothers, BPA40 significantly increased (p<0.05) total and endometrial layers, while BPA80 and BPA200 reduced all uterine layers. In offspring, BPA40 reduced all uterine layers, while BPA80 showed no significant difference and BPA200 reduced endometrium and increased myometrium layers. Conclusions: BPA did not affect the number and gender of pups per litter, but caused malformation and disturbed sex differentiation. Adult and fetal exposure to BPA differently affected the uterine layers of vesper mice. This study showed that adult and in utero exposure to BPA has long-term effects in this species and these alterations might affect fertility of the animals. Such findings offer valuable insight of BPA effects in women.
- Affiliations
Universidade Federal do Rio Grande Instituto de Ciências Fisiológicas
Citations
de Miranda Vasconcellos Vilela, J. (2020). Effect of exposure to Bisphenol A in the thickness of uterine layers in the vesper mouse. The 19th World Congress of the International Society of Gynecological Endocrinology, Online. https://hdl.handle.net/2078.5/106663