Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by commercial assays
(2017) Clinical Microbiology and Infection — Vol. 23, n° 4, p. 267 (2017)
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- Abstract
- Objectives: Neither the liquid medium-based Bactec MGIT, nor commercial molecular assays such as the Xpert MTB/RIF and the MTBDR. plus V2.0 assays are capable of detecting up to 30% of rifampicin-resistant Mycobacterium tuberculosis strains in Swaziland because of the large proportion of the rpoB Ile491Phe mutations. In other countries, the frequency of this mutation is thought to be low. Methods: We designed a real-time multiplex allele-specific PCR assay to identify the rpoB Ile491Phe mutation responsible for these undetected resistant M. tuberculosis strains. Results: The technique showed 100% similarity with rpoB sequencing on a panel of 78 strains from Swaziland. Conclusions: We propose that the detection of the rpoB Ile491Phe rpoB mutation should complement commercial assays for the diagnosis of rifampicin-resistant M. tuberculosis in routine conditions, particularly in countries where this specific mutation is frequent. The technique proposed in this paper is adapted for most reference laboratories. © 2016 The Author(s).
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Citations
André, E., Goeminne, L., Colmant, A., Beckert, P., Niemann, S., & Delmée, M. (2017). Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by commercial assays. Clinical Microbiology and Infection, 23(4), 267. https://doi.org/10.1016/j.cmi.2016.12.009 (Original work published 2017)