Characterization of in vitro growth of bovine preantral ovarian follicles: A preliminary study.
(1993) Theriogenology — Vol. 39, n° 4, p. 811-821 (1993)
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- Abstract
- Preantral follicles were mechanically extracted from bovine ovaries collected at slaughter. On average 90 early growing follicles were collected per ovary. The follicles were cultured for 7 days in Dulbecco's modified Eagle medium F-12 nutrient mixture + 10% fetal calf serum + 10% newborn calf serum. The individual follicular diameter and the follicular DNA content were recorded at the start and the end of culture. The follicular DNA content was estimated by a microfluorometric method using the fluorochrome, 4'-6 diamidino-2-phenylindol-2HCl (DAPI). After culture, 86% of the follicles looked morphologically normal. Of the surviving follicles, 73% showed an increase in diameter varying between 5 and 30 mum, and the follicular DNA content increased from 1 to 72% under the same culture conditions. These results indicate that bovine preantral follicles can survive in vitro and even grow in a single medium.
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Nuttinck, F., Mermillod, P., Massip, A., & Dessy, F. (1993). Characterization of in vitro growth of bovine preantral ovarian follicles: A preliminary study. Theriogenology, 39(4), 811-821. https://doi.org/10.1016/0093-691X(93)90420-A (Original work published 1993)