Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP
(2009) Acta Crystallographica Section F-structural Biology And Crystallization Communications — Vol. 65, p. 102-104 (2009)
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- Abstract
- Interleukin-22 (IL-22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL-22 was incubated with its soluble decoy receptor IL-22BP (IL-22 binding protein) and the IL-22 -IL-22BP complex was crystallized in hanging drops using the vapour-diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 angstrom resolution. The crystal belonged to the tetragonal space group P41, with unit-cell parameters a = b = 67.9, c = 172.5 angstrom, and contained two IL-22-IL- 22BP complexes per asymmetric unit.
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Watanabe, L., de Moura, P. R., Nascimento, A. S., Colau, D., Dumoutier, L., Renauld, J.-C., & Polikarpov, I. (2009). Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP. Acta Crystallographica Section F-structural Biology And Crystallization Communications, 65, 102-104. https://doi.org/10.1107/S1744309108042309 (Original work published 2009)