Turbidimetric latex immunoassay of placental lactogen on microtiter plates.

Collet-Cassart, D;Limet, J N;Van Krieken, L;De Hertogh, René
(1989) Clinical Chemistry : international journal of molecular diagnostics and laboratory medicine — Vol. 35, n° 1, p. 141-143 (1989)

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Authors
  • Collet-Cassart, D
    Author
  • Limet, J N
    Author
  • Van Krieken, L
    Author
  • De Hertogh, RenéUCLouvain
    Author
Abstract
In this latex immunoassay for human placental lactogen, microtiter plates are used as the reaction vessel and the absorbance at 405 nm is measured to quantify the reactions. This 30-min assay necessitates only one serum dilution and two pipetting steps. The calibration curve extends from 0.5 to 15 mg/L. CVs range from 4.2% to 7.0% for within-run determination and from 7.0% to 11.2% for between-run determinations. A correlation coefficient of 0.949 was obtained for 84 sera when the method was compared with a commercial radioimmunoassay.
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Collet-Cassart, D., Limet, J. N., Van Krieken, L., & De Hertogh, R. (1989). Turbidimetric latex immunoassay of placental lactogen on microtiter plates. Clinical Chemistry : international journal of molecular diagnostics and laboratory medicine, 35(1), 141-143. https://hdl.handle.net/2078.5/41538 (Original work published 1989)