Protein-loaded poly(epsilon-caprolactone) microparticles III. Entrapment of superoxide dismutase by the (water-in-oil)-in water solvent evaporation method
(1999) STP Pharma Sciences — Vol. 9, n° 2, p. 175-181 (1999)
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- This work investigated some process parameters involved in the entrapment of superoxide dismutase (model protein) in poly(epsilon-caprolactone) microparticles by a multiple emulsion solvent evaporation method. In optimal operating conditions, the particles were smooth and spherical (mean diameter 8.66 +/- 0.18 mu m) with entrapment efficiency and retained biological activity of 68 +/- 2% and 8.48 +/- 1.07% (n = 3), respectively. This dramatic activity loss was ascribed to the deleterious effect of the polymer solvent. The use of alpha-cyclodextrin decreased the entrapment efficiency and retained biological activity. These two parameters were not considerably influenced by the freeze-drying. Moreover, thermal analysis showed that superoxide dismutase did not form a true solution with the polymer and an increase in the polymer crystallization temperature after the entrapment process.
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Youan, B., Gillard, J., & Rollmann, B. (1999). Protein-loaded poly(epsilon-caprolactone) microparticles III. Entrapment of superoxide dismutase by the (water-in-oil)-in water solvent evaporation method. STP Pharma Sciences, 9(2), 175-181. https://hdl.handle.net/2078.5/140535 (Original work published 1999)