Health beneficial effects of carotenoids & polyphenols from locally grown fruits & vegetables determined by in-vitro assessment of Caco-2 cells following simulated gastro-intestinal digestion
(2017)
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- Abstract
- A diet rich in fruits and vegetables and therefore in phytochemicals has been frequently proposed for a healthy living and the prevention of a number of chronic diseases. Carotenoids and polyphenols constitute 2 important classes of phytochemicals, possessing anti-oxidant and anti-inflammatory properties, and have been linked to the reduced incidence of e.g. cardiovascular disease or cancer. Within the proposed study, we focussed on the characterization of local plums and cabbage varieties, rich in carotenoids and polyphenols, their bioavailability, and aspects related to inflammatory responses as occurring in inflammatory bowel diseases (IBD). As the original polyphenol and carotenoid composition undergoes changes during gastrointestinal digestion, changes of their profiles from the native occurrence to the fraction available for absorption has been studied. Digesta rich in predominant polyphenols and carotenoids were then further studied by means of an epithelial model, i.e. Caco-2 cells (monoculture) and in conjunction with mucus producing HT-29 MTX cells (coculture) and THP-1 like macrophages (triple-culture), in terms of studying cellular uptake and their effects to reduce stimulated intestinal inflammation and their effect on the transcriptome and the proteome. While carotenoids were higher in certain brassica than plum varieties, higher concentrations of polyphenols were encountered in certain plums. This changed dramatically following digestion, were most polyphenols were no longer detectable, possibly due to the specifics of the digestion system, including milk to facilitate carotenoid bioaccessibility. Though carotenoid bioaccessibility from this system was not very high, most carotenoids were detectable following simulated gastro-intestinal digestion. Both plum and cabbage varieties significantly reduced certain markers of oxidative stress (Nrf2) and inflammation (IL-6, IL-8 and NF-κB) and showed an impact on different important antioxidant enzymes, e.g. glutathione transferase, SOD and catalase, though these effects were moderate and could not be aligned to carotenoids or polyphenols. In conclusion, though plum and cabbage varieties showed promise regarding a potential intervention for IBD subjects, these effects could not be allocated to major carotenoids and polyphenols alone.
- Affiliations
UCLouvain SST/ISV - Institut des sciences de la vie UCLouvain SST/LIBST - Louvain Institute of Biomolecular Science and Technology
Citations
Kaulmann, A. (2017). Health beneficial effects of carotenoids & polyphenols from locally grown fruits & vegetables determined by in-vitro assessment of Caco-2 cells following simulated gastro-intestinal digestion. https://hdl.handle.net/2078.5/177593