Fibrin hydrogels as Stem Cells of the Apical Papilla (SCAP) Scaffold for regenerative medicine

Germain, Loïc;De Berdt, Pauline;Vanacker, Julie;Leprince, Julian;des Rieux, Anne;et.al.
(2015) Regenerative Medicine — Vol. 10, n° 2, p. 153-167 (2015)

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Abstract
Evaluation of survival, proliferation and neurodifferentiation of dental stem cells from the apical papilla (SCAP) in fibrin hydrogels. We hypothesized that fibrin composition will influence cell behavior. Methods: Modulus, pore and fiber size were measured. SCAP in vitro viability, proliferation and neural differentiation, as well as in vivo proliferation and angiogenesis were studied. Results: Hydrogel moduli were influenced by fibrin formulation but not hydrogel morphology, SCAP in vitro viability and proliferation. In total 60% of SCAP expressed PanNeurofilament in vitro without induction in Fibrinogen50-Thrombin10. SCAP proliferated when implanted in vivo and stimulated host endothelial cell infiltration. Conclusion: Fibrinogen30-Thrombin10 or Thrombin50 would be more favorable to in vitro SCAP viability and in vivo proliferation, while Fibrinogen50-Thrombin50 would be more adapted to neurodifferentiation
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Germain, L., De Berdt, P., Vanacker, J., Leprince, J., Diogenes, A., Jacobs, D., Vandermeulen, G., Bouzin, C., Préat, V., Dupont, C., & des Rieux, A. (2015). Fibrin hydrogels as Stem Cells of the Apical Papilla (SCAP) Scaffold for regenerative medicine. Regenerative Medicine, 10(2), 153-167. https://doi.org/10.2217/RME.14.81 (Original work published 2015)