IL-23 and IL-12 have overlapping, but distinct, effects on murine dendritic cells.
(2002) Journal of Immunology — Vol. 168, n° 11, p. 5448-5454 (2002)
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- Abstract
- IL-23 is a recently discovered heterodimeric cytokine that shares biological properties with proinflammatory cytokines. The biologically active heterodimer consists of p19 and the p40 subunit of IL-12. IL-23 has been shown to possess biological activities on T cells that are similar as well distinct from those of IL-12. We have constructed single-chain IL-23 and IL-12 fusion proteins (IL-23-Ig and IL-12-Ig) and have compared the two recombinant proteins for effects on murine dendritic cells (DC). Here we show that the IL-23-Ig can bind a significant proportion of splenic DC of both the CD8alpha(-) and CD8alpha(+) subtypes. Furthermore, IL-23and IL-12-Ig exert biological activities on DC that are only in part overlapping. While both proteins induce IL-12 production from DC, only IL-23-Ig can act directly on CD8alpha(+) DC to promote immunogenic presentation of an otherwise tolerogenic tumor peptide. In addition, the in vitro effects of IL-23-Ig did not appear to require IL-12Rbeta2 or to be mediated by the production of IL-12. These data may establish IL-23 as a novel cytokine with major effects on APC.
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Belladonna, M. L., Renauld, J.-C., Bianchi, R., Vacca, C., Fallarino, F., Orabona, C., Fioretti, M. C., Grohmann, U., & Puccetti, P. (2002). IL-23 and IL-12 have overlapping, but distinct, effects on murine dendritic cells. Journal of Immunology, 168(11), 5448-5454. https://doi.org/10.4049/jimmunol.168.11.5448 (Original work published 2002)