Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures.
(2002) Journal of Clinical Microbiology — Vol. 40, n° 4, p. 1514-1517 (2002)
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- Abstract
- A triplex PCR targeting the 16S rRNA, mecA, and nuc genes was developed for identification of staphylococci and detection of methicillin resistance. After validation of the assay with a collection of strains of staphylococci and enterococci (n = 169), the assay was evaluated with cultures of blood with gram-positive cocci from 40 patients. Accurate results were obtained for 59 (98%) of 61 cultures within 6 h of growth detection.
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Maes, N., Magdalena, J., Rottiers, S., De Gheldre, Y., & Struelens, M. J. (2002). Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures. Journal of Clinical Microbiology, 40(4), 1514-1517. https://doi.org/10.1128/jcm.40.4.1514-1517.2002 (Original work published 2002)