In Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media
(2011) The Journal of Eukaryotic Microbiology — Vol. 58, n° 5, p. 424-425 (2011)
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- Abstract
- Polymyxa betae is a soil-borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus. Sugar beet hairy roots, transformed by Agrobacterium rhizogenes, were inoculated with surface-sterilized root fragments infected by P. betae. After 10 wk in a liquid medium, typical structures of P. betae were observed in this in vitro system. This first in vitro culture of P. betae in liquid medium will contribute to a better understanding of this protist's biology through providing a way to conserve and produce purified isolates of the protist.
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Citations
Desoignies, N., & Legrève, A. (2011). In Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media. The Journal of Eukaryotic Microbiology, 58(5), 424-425. https://doi.org/10.1111/j.1550-7408.2011.00563.x (Original work published 2011)