Assessing sub-cellular resolution in spatial proteomics experiments

Gatto, Laurent;Breckels, Lisa M.;Lilley, Kathryn S.
(2019) Current Opinion in Chemical Biology — Vol. 48, p. 123-149 (2019)

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  • Gatto, LaurentUCLouvain
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  • Breckels, Lisa M.
    Author
  • Lilley, Kathryn S.
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Abstract
The sub-cellular localisation of a protein is vital in defining its function, and a protein's mis-localisation is known to lead to adverse effect. As a result, numerous experimental techniques and datasets have been published, with the aim of deciphering the localisation of proteins at various scales and resolutions, including high profile mass spectrometry-based efforts. Here, we present a meta-analysis assessing and comparing the sub-cellular resolution of 29 such mass spectrometry-based spatial proteomics experiments using a newly developed tool termed QSep. Our goal is to provide a simple quantitative report of how well spatial proteomics resolve the sub-cellular niches they describe to inform and guide developers and users of such methods.
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Gatto, L., Breckels, L. M., & Lilley, K. S. (2019). Assessing sub-cellular resolution in spatial proteomics experiments. Current Opinion in Chemical Biology, 48, 123-149. https://doi.org/10.1016/j.cbpa.2018.11.015 (Original work published 2019)