Simultaneous detection and quantification of acidic and basic drugs in serum toxicological screening: use of ion-pairing technique in liquid-liquid extraction
(1999) Acta clinica Belgica. Supplementum — Vol. 53, n° Suppl 1, p. 74-78 (1999)
Files
26230.pdf
Restricted Access - Adobe PDF
- 2.31 MB
Details
- Authors
- Abstract
- The toxicological screening, using the combination of high performance liquid chromatography with diode array ultraviolet detector and ion-pairing technique in liquid-liquid extraction, is an effective tool in the identification and quantification of the acidic and basic substances in a single run. The use of an ion-pairing technique in the conventional extraction shows the co-extraction of the uncharged and charged form of the analytes present in a serum sample. The stationary phase used is C-18-bonded phase. The mobile phase is acetonitrile--phosphate buffer (pH 3; 25 mM) containing 25 mM triethylammonium as ion-pairing agent. The analytical validation shows reproducible recoveries, good day-to-day repeatability and sensible results compatible with clinical and forensic use.
- Affiliations
Citations
Brau, M., Vanbinst, R., Hassoun, A., & Wallemacq, P. (1999). Simultaneous detection and quantification of acidic and basic drugs in serum toxicological screening: use of ion-pairing technique in liquid-liquid extraction. Acta clinica Belgica. Supplementum, 53(Suppl 1), 74-78. https://hdl.handle.net/2078.5/133346 (Original work published 1999)