Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study.
(2009) International Journal of Colorectal Disease : clinical and molecular gastroenterology and surgery — Vol. 24, n° 2, p. 129-138 (2008)
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- Abstract
- AIMS: The aims of the study were to study the effect of pre-operative treatment on the expression of tumour-related proteins and to correlate the expression of these proteins with response and survival of patients with advanced rectal cancer. MATERIALS AND METHODS: Tissue micro-arrays from pre- and post-treatment biopsies of 99 patients with rectal cancer treated with pre-operative (chemo)radiotherapy were stained for epidermal growth factor receptor (EGFR), carbonic anhydrase IX, Ki67, vascular endothelial growth factor, cyclo-oxygenase 2 (COX-2) and cleaved cytokeratin 18 (c-CK18). Also, fibro-inflammatory alterations after treatment were evaluated. RESULTS: Pre-operative (chemo)radiotherapy caused fibro-inflammatory changes, a downregulation of proliferation (Ki67) and EGFR and an upregulation of apoptosis (cleaved CK18). Patients with a good regression during pre-operative treatment showed less proliferating and apoptotic cells in the resection specimen. Multivariate analysis showed that T downstaging, fibro-inflammatory changes in the resection specimen and COX-2 expression in the biopsy correlated with overall survival. CONCLUSIONS: Pre-operative treatment has an effect on proliferation, apoptosis, inflammation and EGFR expression. The classical clinical parameters as well as fibro-inflammatory changes and COX-2 expression seem most valuable as predictors for survival.
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Citations
Debucquoy, A., Goethals, L., Libbrecht, L., Perneel, C., Geboes, K., Ectors, N., McBride, W. H., & Haustermans, K. (2009). Molecular and clinico-pathological markers in rectal cancer: a tissue micro-array study. International Journal of Colorectal Disease : clinical and molecular gastroenterology and surgery, 24(2), 129-138. https://doi.org/10.1007/s00384-008-0608-8 (Original work published 2008)