Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40
(1990) Cytokine — Vol. 2, n° 1, p. 9-12 (1990)
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- Authors
Van Snick, Jacques 
- Abstract
- A cDNA encoding the human homolog of mouse T-cell and mast cell growth factor P40 was derived from peripheral blood mononuclear cells (PBMC) stimulated with phytohemagglutinin and phorbol myristate acetate. Sequence analysis of the cDNA predicted a precursor protein of 144 amino acids including a signal peptide of 18 residues, a structure identical with that of mouse P40. The homology between the mouse and human proteins is 55% with a perfect conservation of the 10 cysteine residues present in the mature polypeptide. Expression of the cDNA for human P40 in a baculovirus vector yielded a protein capable of enhancing in vitro survival of human T cell lines.
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Citations
Renauld, J.-C., Goethals, A., Houssiau, F., Van Roost, E., & Van Snick, J. (1990). Cloning and expression of a cDNA for the human homolog of mouse T cell and mast cell growth factor P40. Cytokine, 2(1), 9-12. https://doi.org/10.1016/1043-4666(90)90037-T (Original work published 1990)