Evaluation of arbitrarily primed polymerase chain reaction analysis for typing Legionella pneumophila.
(1998) Clinical Microbiology and Infection — Vol. 4, n° 3, p. 149-154 (1998)
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- OBJECTIVE: To evaluate the performance of arbitrarily primed polymerase chain reaction (AP-PCR) analysis in epidemiologic typing of Legionella pneumophila. METHODS: Sixty-two isolates of L. pneumophila of serogroups 1, 3, 6 and 10, including epidemiologically related and unrelated isolates, were analyzed by AP-PCR using the primer BG2. Twenty-six of the serogroup 1 isolates were typed by pulsed-field gel electrophoresis (PFGE). RESULTS: AP-PCR analysis showed 98% typeability and complete reproducibility. A majority of unrelated isolates of each serogroup could be distinguished (discrimination index: 92%). Clinical isolates showed AP-PCR patterns indistinguishable from those of the isolates of the related environmental source. PFGE and AP-PCR results were in agreement for 88% of isolates. CONCLUSIONS: Single-primer AP-PCR analysis can be used as a simple and reproducible screening method for typing L. pneumophila strains of different serogroups.
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Maes, N., Wauters, G., & Struelens, M. J. (1998). Evaluation of arbitrarily primed polymerase chain reaction analysis for typing Legionella pneumophila. Clinical Microbiology and Infection, 4(3), 149-154. https://doi.org/10.1111/j.1469-0691.1998.tb00378.x (Original work published 1998)