Validation of a method to detect cocaine and benzoylecgonine in human whole blood by gas chromatography-mass spectrometry and application in body packers and stuffers cases in the North of Chile

Bravo, Felipe;Contzen, María Cecilia;Mollo, Julio;Calderon, Pedro Buc;Benites, Julio
(2012) Journal of the Chilean Chemical Sociaty — Vol. 57, n° 3, p. 1253-1255 (2012)

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Authors
  • Bravo, Felipe
    Author
  • Contzen, María Cecilia
    Author
  • Mollo, Julio
    Author
  • Calderon, Pedro Buc
    Author
  • Benites, Julio
    Author
Abstract
The present paper describes a method for the simultaneous determination of cocaine and benzoylecgonine in human whole blood. The drugs were extracted with phosphate buffer at pH 6, followed by solid-phase extraction and quantification by GC/MS with electron impact ionization using helium as carrier gas. Quantification was performed using cocaine-d3 as internal standard in selected ion monitoring mode. The method is very simple, rapid and sensitive. The specificity, linearity, intra- and inter-assay precision and accuracy, and extraction recovery were fully evaluated. The limits of detection were 3.6 ng/mL for cocaine and 6.8 ng/mL for benzoylecgonine. The method was applied to blood samples removed at autopsy from body packers and stuffers cases in the I - IV and XV regions of Chile during 2008-2010.

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Bravo, F., Contzen, M. C., Mollo, J., Calderon, P. B., & Benites, J. (2012). Validation of a method to detect cocaine and benzoylecgonine in human whole blood by gas chromatography-mass spectrometry and application in body packers and stuffers cases in the North of Chile. Journal of the Chilean Chemical Sociaty, 57(3), 1253-1255. https://hdl.handle.net/2078.5/89941 (Original work published 2012)