Bacillus thuringiensis is a sporulating bacterium, which produces parasporal inclusions toxic to insects. Widely used methodologies to isolate Bt from soil consist of a thermal shock treatment followed by selective germination of spores. The results presented here suggest that a preliminary 5 h dry-heat treatment largely enhance the selectivity of these procedures.
Affiliations
Universidad Simon BolivarLab. de Biotecnología Agrícola, Dpto. de Biología Celular
Citations
APA
Chicago
FWB
Santana, M. A., Moccia-V, C. C., & Gillis, A. (2008). Bacillus thuringiensis improved isolation methodology from soil samples. Journal of Microbiological Methods, 75(2), 357-358. https://doi.org/10.1016/j.mimet.2008.06.008 (Original work published 2008)